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KMID : 0385219940040010039
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Korean Journal of Gerontology
1994 Volume.4 No. 1 p.39 ~ p.45
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Study on Anti-aging Action of Docosahexaenoic Acid (DHA) in Rats 2. Effect of docosahexaenoic Acid (DHA)-Rich Fish Oil on Oxygen Radicals and Their Scavenger Enxzymes in Rats
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Choi Jin-Ho
Kim Jae-Il
Kim Dong-Woo
Moon Young-Sil
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Abstract
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Previous studies have shown that docosahexaenoic acid in diets resulted in reductions of total and LDL cholesterols and increases of EPA/AA and DHA/AA ratios in werum and liver membranes. We wished to investigate whether docosahexaenoic acid would be resulted in the incorporation of docosahexaenoic acid into atherogenic index, reactive oxygen species(ROS), their scavenger enzymes on free radical in serum and liver membranes of rats Following DHA-rich fish oil administration for 10 weeks, there were 13-18% fall in triglycerides, and 13-28% fall LDL cholesterols in serum. Cholesterols in liver membranes also significantly decreased with the administration of DHA-rich fish oil. Atherogenic index significantly decreased from 3.43 to 2.96-3.28(p<0.001 in P/S ratio 1.2-1.6)by the administration of DHA-rich fish oil.
DHA-rich fish oil led to increases of EPA(20:5) and DHA (22:6), and reductions of linoleic acid(18:2), but arachidonic acid(20:4) almost did not change. DHA-rich fish oil also led to increases of the ratios of 20:5/20:4, 22:6/20:4, and n-3/n-6(p<0.001). Lipid peroxides in serum and liver membranes were markedly increased by administration of DHA-rich fish oil, but DHA-rich fish oil resulted in remarked decreases of hydroxyl radical and hydrogen peroxide in liver microsomes, and hydrogen peroxide in mitochondria. Superoxide dismutase activity in liver mitochondria and glutathione peroxidase activity in liver cytosol were significantly increased by administration of DHA-rich fish oil. These results suggest that docosahexaenoic acid may be due to inhibitions of atherogenic index and free radical formations with less total and LDL cholesterols, hydroxyl radical and hydrogen peroxide, and a larger scavenger enzyme activity on free radical, resulting in a greater reduction of cardiovascular disease, and intervention of aging process.
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KEYWORD
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docosahexaenoic acid(DHA), atherogenic index, reactive oxygen species(ROS), scavenger enzyme, superoxide dismutase (SOD)
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